Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
* Modification: Replace thiosulfate with 5.00 g/l tetrathionate (K2S4O6) added from a stock solution sterilized by filtration.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 3.00 | g | 3 | 22.045 | |
MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 | |
(NH4)2SO4 | 3.00 | g | 3 | 22.703 | |
CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 | |
Na2S2O3 x 5 H2O* | 5.00 | g | 5 | 20.146 | |
K2S4O6* | 5.00 | g | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Prepare the medium without thiosulfate, adjust pH to 4.4 - 4.7 and autoclave at 121°C for 15 min. Sterilize thiosulfate separately by filtration and add after autoclaving. | |||||
2 Note: Incubate statically without shaking. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 4.4 - 4.7 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
K2S4O6 | 5 | - |
(NH4)2SO4 | 3 | 22.703 |
KH2PO4 | 3 | 22.045 |
MgSO4 x 7 H2O | 0.5 | 2.029 |
CaCl2 x 2 H2O | 0.25 | 1.701 |
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